PerR - regulated manganese ion uptake contributes to oxidative stress 1 defense in an oral streptococcus
نویسندگان
چکیده
3 Xinhui Wang, Huichun Tong, Xiuzhu Dong 4 5 1, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese 6 Academy of Sciences, Beijing 100101, China 7 2, University of Chinese Academy of Sciences, Beijing 100049, China 8 9 10 11 Running title: PerR-regulated Mn uptake confers H2O2 resistance 12 13 14 15 16 17 18 19 Corresponding author. State Key Laboratory of Microbial Resources, Institute of 20 Microbiology, Chinese Academy of Sciences, No. 1 West Beichen Road, Chaoyang 21 district, Beijing 100101, China. Tel: 86-10-64807567; Fax: 86-10-64807429; E-mail: 22 [email protected] (HC Tong). 23 AEM Accepts, published online ahead of print on 31 January 2014 Appl. Environ. Microbiol. doi:10.1128/AEM.00064-14 Copyright © 2014, American Society for Microbiology. All Rights Reserved.
منابع مشابه
PerR-regulated manganese ion uptake contributes to oxidative stress defense in an oral streptococcus.
Metal homeostasis plays a critical role in antioxidative stress. Streptococcus oligofermentans, an oral commensal facultative anaerobe lacking catalase activity, produces and tolerates abundant H2O2, whereas Dpr (an Fe(2+)-chelating protein)-dependent H2O2 protection does not confer such high tolerance. Here, we report that inactivation of perR, a peroxide-responsive repressor that regulates zi...
متن کاملThe regulator PerR is involved in oxidative stress response and iron homeostasis and is necessary for full virulence of Streptococcus pyogenes.
Ferric uptake regulator (Fur) and Fur-like proteins form an important family of transcriptional regulators in many bacterial species. In this work we have characterized a Fur-like protein, the peroxide regulator PerR, in an M1 serotype of Streptococcus pyogenes. To determine the role of PerR in S. pyogenes, we inactivated the gene by allelic replacement. PerR-deficient bacteria showed 48% reduc...
متن کاملPeroxide Responsive Regulator PerR of group A Streptococcus Is Required for the Expression of Phage-Associated DNase Sda1 under Oxidative Stress
The peroxide regulator (PerR) is a ferric uptake repressor-like protein, which is involved in adaptation to oxidative stress and iron homeostasis in group A streptococcus. A perR mutant is attenuated in surviving in human blood, colonization of the pharynx, and resistance to phagocytic clearance, indicating that the PerR regulon affects both host environment adaptation and immune escape. Sda1 i...
متن کاملIn Staphylococcus aureus, fur is an interactive regulator with PerR, contributes to virulence, and Is necessary for oxidative stress resistance through positive regulation of catalase and iron homeostasis.
The Staphylococcus aureus genome encodes three ferric uptake repressor (Fur) homologues: Fur, PerR, and Zur. To determine the exact role of Fur in S. aureus, we inactivated the fur gene by allelic replacement using a tetracycline resistance cassette, creating strain MJH010 (fur). The mutant had a growth defect in rich medium, and this defect was exacerbated in metal-depleted CL medium. This gro...
متن کاملPeroxide stimulon and role of PerR in group A Streptococcus.
We have characterized group A Streptococcus (GAS) genome-wide responses to hydrogen peroxide and assessed the role of the peroxide response regulator (PerR) in GAS under oxidative stress. Comparison of transcriptome changes elicited by peroxide in wild-type bacteria with those in a perR deletion mutant showed that 76 out of 237 peroxide-regulated genes are PerR dependent. Unlike the PerR-mediat...
متن کامل